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htlr2  (InvivoGen)


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    Structured Review

    InvivoGen htlr2
    Human milk synergizes with LPS to induce TLR4 activation in HEK-hTLR4 cells. ( a ) HEK-hTLR4 cells were incubated overnight with 333 ng/mL LPS ( n = 6), 10% HM ( n = 44), or 10% HM + 333 ng/mL LPS ( n = 44) and TLR4-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + LPS conditions represents a HM sample from a single individual. Data are represented as LPS equivalents and normalized to LPS only response. ( b ) <t>HEK-hTLR2</t> cells were incubated overnight with 12.5 µg/mL Pam3CSK4 ( n = 6), 10% HM ( n = 44), or 10% HM + 12.5 µg/mL Pam3CSK4 ( n = 44) and TLR2-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + Pam3CSK4 conditions represents a HM sample from a single individual. Data are represented as Pam3CSK4 equivalents and normalized to Pam3CSK4 only response. Box indicates interquartile range and median. Whiskers extend from minimum to maximum. **** P < 0.0001. NS, not significant. LPS, lipopolysaccharide. HM, human milk. Pam3CSK4, Pam3CysSerLys4.
    Htlr2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htlr2/product/InvivoGen
    Average 93 stars, based on 8 article reviews
    htlr2 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Human milk affects TLR4 activation and LPS-induced inflammatory cytokine expression in Caco-2 intestinal epithelial cells"

    Article Title: Human milk affects TLR4 activation and LPS-induced inflammatory cytokine expression in Caco-2 intestinal epithelial cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-64000-z

    Human milk synergizes with LPS to induce TLR4 activation in HEK-hTLR4 cells. ( a ) HEK-hTLR4 cells were incubated overnight with 333 ng/mL LPS ( n = 6), 10% HM ( n = 44), or 10% HM + 333 ng/mL LPS ( n = 44) and TLR4-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + LPS conditions represents a HM sample from a single individual. Data are represented as LPS equivalents and normalized to LPS only response. ( b ) HEK-hTLR2 cells were incubated overnight with 12.5 µg/mL Pam3CSK4 ( n = 6), 10% HM ( n = 44), or 10% HM + 12.5 µg/mL Pam3CSK4 ( n = 44) and TLR2-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + Pam3CSK4 conditions represents a HM sample from a single individual. Data are represented as Pam3CSK4 equivalents and normalized to Pam3CSK4 only response. Box indicates interquartile range and median. Whiskers extend from minimum to maximum. **** P < 0.0001. NS, not significant. LPS, lipopolysaccharide. HM, human milk. Pam3CSK4, Pam3CysSerLys4.
    Figure Legend Snippet: Human milk synergizes with LPS to induce TLR4 activation in HEK-hTLR4 cells. ( a ) HEK-hTLR4 cells were incubated overnight with 333 ng/mL LPS ( n = 6), 10% HM ( n = 44), or 10% HM + 333 ng/mL LPS ( n = 44) and TLR4-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + LPS conditions represents a HM sample from a single individual. Data are represented as LPS equivalents and normalized to LPS only response. ( b ) HEK-hTLR2 cells were incubated overnight with 12.5 µg/mL Pam3CSK4 ( n = 6), 10% HM ( n = 44), or 10% HM + 12.5 µg/mL Pam3CSK4 ( n = 44) and TLR2-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + Pam3CSK4 conditions represents a HM sample from a single individual. Data are represented as Pam3CSK4 equivalents and normalized to Pam3CSK4 only response. Box indicates interquartile range and median. Whiskers extend from minimum to maximum. **** P < 0.0001. NS, not significant. LPS, lipopolysaccharide. HM, human milk. Pam3CSK4, Pam3CysSerLys4.

    Techniques Used: Activation Assay, Incubation, SEAP Assay



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    Image Search Results


    Human milk synergizes with LPS to induce TLR4 activation in HEK-hTLR4 cells. ( a ) HEK-hTLR4 cells were incubated overnight with 333 ng/mL LPS ( n = 6), 10% HM ( n = 44), or 10% HM + 333 ng/mL LPS ( n = 44) and TLR4-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + LPS conditions represents a HM sample from a single individual. Data are represented as LPS equivalents and normalized to LPS only response. ( b ) HEK-hTLR2 cells were incubated overnight with 12.5 µg/mL Pam3CSK4 ( n = 6), 10% HM ( n = 44), or 10% HM + 12.5 µg/mL Pam3CSK4 ( n = 44) and TLR2-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + Pam3CSK4 conditions represents a HM sample from a single individual. Data are represented as Pam3CSK4 equivalents and normalized to Pam3CSK4 only response. Box indicates interquartile range and median. Whiskers extend from minimum to maximum. **** P < 0.0001. NS, not significant. LPS, lipopolysaccharide. HM, human milk. Pam3CSK4, Pam3CysSerLys4.

    Journal: Scientific Reports

    Article Title: Human milk affects TLR4 activation and LPS-induced inflammatory cytokine expression in Caco-2 intestinal epithelial cells

    doi: 10.1038/s41598-024-64000-z

    Figure Lengend Snippet: Human milk synergizes with LPS to induce TLR4 activation in HEK-hTLR4 cells. ( a ) HEK-hTLR4 cells were incubated overnight with 333 ng/mL LPS ( n = 6), 10% HM ( n = 44), or 10% HM + 333 ng/mL LPS ( n = 44) and TLR4-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + LPS conditions represents a HM sample from a single individual. Data are represented as LPS equivalents and normalized to LPS only response. ( b ) HEK-hTLR2 cells were incubated overnight with 12.5 µg/mL Pam3CSK4 ( n = 6), 10% HM ( n = 44), or 10% HM + 12.5 µg/mL Pam3CSK4 ( n = 44) and TLR2-induced NF-κB activation was assessed using a SEAP assay. Each point in the HM and HM + Pam3CSK4 conditions represents a HM sample from a single individual. Data are represented as Pam3CSK4 equivalents and normalized to Pam3CSK4 only response. Box indicates interquartile range and median. Whiskers extend from minimum to maximum. **** P < 0.0001. NS, not significant. LPS, lipopolysaccharide. HM, human milk. Pam3CSK4, Pam3CysSerLys4.

    Article Snippet: The human TLR-transfected cell lines HEK-Blue hTLR4 and HEK-Blue hTLR2 were purchased from Invivogen (San Diego, CA) and maintained in an incubator at 37 °C in 5% CO 2 .

    Techniques: Activation Assay, Incubation, SEAP Assay